SYLABUS
Academic year ……2020/2021
Name of a course/module | Molecular Biology | |
Name of the department where the course is held | Department of Clinical Molecular Biology | |
e-Mail of the department | zkbm@umb.edu.pl | |
Faculty of | Medicine with Division of Dentistry and Division of Medical Education in English | |
Name of a field of study | medicine | |
Level of education | First degree studies; Uniform master’s degree studies | |
Language of instructions | English | |
Form of study |
Full time £ Part time £
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Type of course | Obligatory £ | Facultative £ |
Year of study/semestr |
I £ II £ III £ IV £ V £ VI £
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1 £ 2 £ 3 £ 4 £ 5 £ 6 £ 7 £ 8 £ 9£ 10 £ 11 £ 12 £
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Introductory courses with preliminary requirements | The implementation of learning outcomes in terms of knowledge, skills and competences of the previous years of study, particucalrly biology and biochemistry | |
Number of didactic hours with specification of form of conducting classes | 15 hours in this: 5 – lectures; 10 - seminars | |
Assumptions and aims of the course | The aim of the course is to acquaint the graduate with the organization of human genes and genome, molecular mechanisms of genome stability and variability, the process of the expression of the genomic information, methods that are used in molecular analysis, and the applications of the knowledge in medical practice. | |
Didactic methods |
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Full name of the person conducting the course | PhD Oksana Kowalczuk | |
Full name of the person responsible for teaching |
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Symbol and number of learning outcomes according to the teaching standards and other learning outcomes | Description of directional learning outcomes | Verification methods for achieving intended learning outcome |
| Knowledge |
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BW13 | The graduate knows nucleotides’ functions in the cell, primary and secondary structure of DNA and RNA and the structure of chromatin;
| Summarizing methods: - a final test in form of multiple choice questions MCO, yes/no answer or answer completion. Forming methods: - self-study and preparation of a presentation on a particular term -answer the questions - continuous assessment by teacher of the graduate’s activity during the discussion (in points from 0 to 3)
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BW14 | The graduate knows functions and basic methods of research of human genome, transcriptome and proteome; describe the process of replication, reconstruction and recombination of DNA, the process of transcription, translation and degradation of DNA, RNA and proteins; concepts of regulation of gene expression;
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CW4 | The graduate has got the knowledge of molecular base of mutagenesis | |
CW9 | The graduate has got the knowledge of elementary diagnostics of gene and chromosome mutation developing hereditary and acquired diseases as well as neoplasm | |
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| Skills | |
BU9 | The g The graduate is skilled at using fundamental laboratory techniques, such as electrophoresis nucleic acids | |
BU11 | The graduate is skilled at using database, including that of internet and getting necessary information by accessible tools | |
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| Social competence | |
K1 | The graduate has got the social competence to be aware of his/her own limitations and need to improve their skills continually | |
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ECTS points | 1 | |
Student’s workload | ||
Form of activity | Number of hours to complete the activity | |
Classes that require the participation of the teacher | ||
| 5 | |
| 10 | |
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| Total hours: 15 | |
Student’s self-study | ||
| 5 | |
| 5 | |
| 5 | |
| Total hours: 15 | |
Course content | |
Learning outcomes (symbol and numer) | Topics |
BW13, BW14, CW4, CW9
BW13, BW14, CW4, CW9, BU9, BU11, K1
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Lectures An introduction in molecular biology: - human genome organization, - molecular mechanisms of genetic stability and variability: DNA replication DNA damage and repair, - genetic variability: molecular mechanisms of mutation emergence, genetic recombination, - molecular mechanisms of gene expression, - regulation of gene expression, - analysis of gene structure and functions – an overview of molecular biology methods, - application of molecular biology knowledge and methods in medicine. Seminars Genetic information and the organization of the human genome. Nucleic acids as a hereditary material: DNA structure and characteristics, RNA structure and kinds. Chromatin and chromosomes. Bacterial genomes. Genomics. DNA replication: molecular mechanisms and proteins and enzymes that participate in the process. Telomeres and telomerase: a chromosomal ends replication problem. Replication inhibitors as anticancer and antiviral drugs. The fidelity of the DNA replication. DNA damage and repair. Basic DNA repair mechanisms. Cellular DNA damage response and its connection with cell cycle and apoptosis. Use of DNA damage factors as anticancer and antiviral drugs. Molecular mechanisms of genetic variability: mutation emergence, genetic recombination). Kinds of mutations. Inherited and somatic mutations. Phenotypic effects of the mutations. Genetic polymorphisms and their impact for complex diseases. Gene expression: molecular mechanisms and participants. An organization of human/eukaryotic genes and bacterial genes (gene promoters, coding part, exons and introns, regulatory sequences). An impact of general transcription factors in eukaryotic gene expression. Regulation of gene expression – a role of transcription factors, chromatin regulation factors, DNA methylation and noncoding regulatory RNAs. Basic molecular biology methods and their application in medical diagnosis and personalized therapy approach. Analysis of gene structure and functions by PCR and hybridization methods. DNA sequencing methods. Modern high throughput methods of analyzing of the genomes, transcriptomes and proteomes. |
Obligatory texbooks |
TA Brown. Genomes, 4e. BIOS Scientific Publisher 2014 LA Allison. Fundamental Molecular Biology, 2e. JohnWilley&Sons, Inc 2011 |
Optional textbook: JD Watson et al. Biology of the gene. Pearson 2013 WS Klug et al. Concepts of Genetics. Pearson 2012 |
Criteria for assessing the achieved learning outcomes and the form and conditions for receiving credit: |
Preparation of a multimedial presentation on a particular topic of the course. Correct answers to at least 60% of the seminar test questions and activity during the discussion. Final written test in the form of answer completion for those students who did not get a credit for the particular seminars and correct answer for at least 60% of the answers. In the excused absence - the obligation to make up classes after consultation with the lecturer.
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Oksana Kowalczuk, PhD
……………………………………………………..
(date and signature of the person preparing the syllabus)
Jacek Nikliński, Professor, PhD, MD Oksana Kowalczuk, PhD
……………………………………………… ………………………………………………
(date and signature of the Head of the and (course coordinator)
Department where the course is held)